Aggregation of Monoclonal Antibody Products: Formation and Removal
Aggregate formation is influenced by multiple aspects of the bioproduction process but can be mitigated by good process design and control. Monoclonal antibody (mAb) products have emerged as an extremely important and valuable class of therapeutic products for the treatment of cancer and other diseases such as rheumatoid arthritis, …
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Selective removal of aggregates with Capto adhere
aggregate removal are required. Capto adhere is a strong ion exchanger with multimodal functionality (Fig 1), which offers a different selectivity compared to traditional ion exchangers. Capto adhere is designed for intermediate purification and polishing of MAbs. Removal of protein A, aggregates, host
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Upgrading a manufactured fine aggregate for use in …
1. Introduction. The shortage of natural sand coupled to environmental restrictions has motivated the search for replacement materials in many parts of the world, in particular to meet the demands from the construction and building industry (Gonçalves et al., 2007, Anand and Reddy, 2014, Cordeiro et al., 2016).Manufactured fine aggregate …
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Simplified strategy for developing purification processes for …
The average DAR and drug load distribution were determined using an analytical hydrophobic interaction chromatography (HIC) method as shown in Figs. 2 and 3. 50 µg of each sample were injected onto the Butyl-NPR column (4.6 mm × 3.5 cm, 2.5 μm) (Tosoh Bioscience, Griesheim, Germany) and were eluted over a linear gradient with …
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